FMOC-ClFluorenylmethyloxycarbonyl Chloride (chloroformate ester)
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In the cases of GLYP and AMPA, the analytes were derivatised with FMOC-Cl prior to SPE (Hanke et al., 2008).
In brief, urine samples were derivatized by FMOC-Cl prior to analysis using high performance liquid chromatography (HPLC: Agilent, 1100) equipped with fluorescence detector (FLD: Agilent, 1046A).
Abbreviations MPP: 3-Methylphosphinico-propionic acid MPA: 2-Methylphosphinico-acetic acid GC: Gas chromatography FPD: Flame photometric detector TMOA: Trimethyl orthoacetate RSD: Relative standard deviations AMPA: Aminomethylphosphonic acid CNBF: 4-Chloro-3,5-dinitrobenzotrifluoride FMOC-Cl: 9-Fluorenylmethylchloroformate FLD: Fluorescence detection MSD: Mass-selective detection LOD: Limit of detection LC: Liquid chromatography isoPCF: Isopropyl chloroformate LOQ: Limit of quantification.