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The results of the SRM analysis showed that the relative peak ratio between the endogenous hepcidin-M and SI-hepcidin-M was 0.01 (50 fmol of serum hepcidin in the 10 [micro]L of serum sample), whereas the relative peak ratio for hepcidin-I could not be determined, indicating that serum hepcidin was detected only with the reduced hepcidin form (Table 3).
Beta cells also consume a lot of oxygen, but if we compare the oxygen consumption rates of beta cells (primary cell: 23 fmol [cell.sup.-1] [h.sup.-1], max.
To confirm the positive identification, the analysis was repeated with the addition of 40 fmol of labeled peptides and the digests desalted with a C18 ZipTip.
For ATV, IC are 1685 and 76.4 fmol per [10.sup.6] cells for 300 mg 13 h and 85 after intake, respectively.
Specific toxicity Toxic content (fmol STX di-HCI Proportions of PST toxin (fmol/cell) equivalent/cell) PSP toxins (%) GTX4 0.6 0.4 1.2 GTX1 0.1 0.1 0.2 dcGTX3 + GTX5 0.4 0.1 0.4 GTX3 0.1 0.05 0.1 dcNeo + Neo 2.0 1.9 5.3 dcSTX 0.4 0.2 0.5 STX 0.2 0.2 0.4 C1 17.6 0.1 0.3 C2 332.1 32.0 91.5 Total 353 35 100 GTX, gonyautotoxins; dc, decarbamoyl; Neo, neosaxitoxin; STX, saxitoxin; C1 and C2, C toxins.
The adduct level, expressed as relative adduct labeling, was converted to fmol adduct per [micro]g DNA as described by Gupta and Dighe (1984).
5 [micro]l of 500 fmol plasmid dsDNA template (pGEM[R]-3Zf(+) control DNA) was used to prepare the sequencing ladders for sizing PCR products (Promega, 2001).
The universal nucleic acid lateral flow assay achieves detection limits of 1.5 to 10 fmol (1.5 to 10 nM).
From this regression the P contents can be estimated as 9.77 [+ or -] 0.70 fmol P/cell for Ochromonas and 0.076 [+ or -] 0.004 fmol P/bacterium for the bacteria (estimate [+ or -] 1 SE).
In the SQ-MRM analysis, we spiked each sample with 50 fmol betaGAL (GDFQFNISR, 542.3/636.3 m/z) during our preparation of the digested sample.