Next, the single clone with both cassette insertions was treated with 1 [micro]g/mL of Dox to remove the neomycin resistance cassette in FNFL flanked by two FRT sites.
Figure S1: detailed procedure of LNL and FNFL sequential and simultaneous targeting.
(B) Sequence of the FNFL targeting region after Dox treatment.
Table S1: the length of the homology arm of LNL and FNFL targeting vectors.
(a) Schematics of sequential targeting LNL and FNFL cassettes to the Eed gene in two steps.
(b) Sequence of the FNFL targeting locus in Eed after Dox treatment.
(b) Schematics of simultaneous LNL and FNFL targeting to the 10 kb regulatory region in one step.
(a) Sequence of the FNFL targeting locus after Dox treatment.