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To reject backscattered and reflected excitation light, a holographic notch-plus band rejection filter (785 nm and 660 nm center wavelength for NIRFI and FRFI, resp.) and a bandpass filter (830 nm and 710 nm center wavelength for NIRFI and FRFI, resp.) were positioned prior to the 50-mm lens.
The reduction of the amplitude in FRFI (77%) was greater than that in NIRFI (54%).
FRFI and NIRFI have been widely used for in vivo animal imaging, since light from longer wavelength, for example, greater than 600 nm, can travel relatively long distances in vivo.
In conclusion, diet-induced background fluorescence acquired using FRFI significantly impacts lymphatic measurement of the fluorescent signal amplitude, but not lymphatic contraction frequency, in the internodal collecting lymphatic vessels in mice fed a regular diet, and skin pigmentation further affects the amplitude.
Caption: Figure 4: Representative fluorescent intensity profiles from NIRFI (black) and FRFI (gray) as a function of time in mice without (a) or with (b) skin pigmentation fed a special diet or in mice without (c) or with (d) skin pigmentation fed a regular diet.
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- Freyre, Gilberto
- Freyssinet International & Cie
- Freyssinet Products Company
- Freyssinet, Eugene
- Freyssinet, Eugène
- Freytag, Gustav
- Frezer Moscow Cutting-Tool Plant