Using data from the optimized and validated marker suite, several molecular diversity indices were calculated for each microsatellite locus using the Microsoft Excel add-in GenAlEx, GenePop, Arlequin, and FSTAT
2.9.3 (Goudet 1995).
, a program to estimate and test gene diversities and fixation indices (version 188.8.131.52).Updated from Goudet.
Pairwise exact genotypic differentiation tests performed by FSTAT
and Genepop showed that--except the pair B, K--all the herds can be treated as separate units, distinct from each other (p<0.05).
Buscando verificar algum padrao de agrupamento entre as populacoes estudadas, foram gerados, com o auxilio do programa FSTAT
versao 184.108.40.206 (GOUDET, 2002), dendrogramas atraves do metodo UPGMA a partir das distancias geneticas de Nei (1972) obtidas entre as populacoes e a estabilidade dos nos foi verificada pelo metodo de reamostragem bootstrap (10.000 repeticoes).
: A Program to Estimate and Test Gene Diversities and Fixation Indices (version 220.127.116.11).
Linkages between each marker were measured using Fstat
v18.104.22.168, and 15 loci were selected (unpublished data).
All parameters were calculated using version 2.9.3 of the FSTAT
Estimates of genetic variation within each year and comparisons between the two colonies were made using the software programs FSTAT
To check for inbreeding in adults (F) and progeny ([F.sub.o]), we used the within population fixation index (F) using the FSTAT
program (Goudet 1995).
Basic descriptive statistics of microsatellite variation, including number of alleles (NA), expected heterozygosity ([H.sub.E], Nei's unbiased gene diversity), and the inbreeding coefficient ([F.sub.IS]), were calculated using FSTAT
All analyses were run using the FSTAT
program, version 22.214.171.124 (Goudet, 1995).
Linkage disequilibrium (LD) was tested in FSTAT
126.96.36.199 (Goudet 2003).