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In order to compare the FTIRM and FTIR results with classical biochemical assays, primary and secondary lipid oxidation products were measured by CD and TBARS, respectively.
However, this result also shows that all phenolic acids and Qc have mild prooxidant effect when used in the absence of [H.sub.2][O.sub.2], in agreement with results obtained by the analysis of FTIRM and FTIR data.
All polyphenolic compounds showed a protective effect in rat enterocytes when administered before and after the oxidative stimulus ([H.sub.2][O.sub.2]), when analyzed by FTIR spectra of single cells (FTIRM) or batch samples, and by classical biochemical methods for lipid oxidation (CD and TBARS).
Abbreviations ATR: Attenuated Total Reflectance CfA: Caffeic acid Cat: Catechin ChA: Chlorogenic acid Cap: Capsaicin FTIR: Fourier Transform Infrared Spectroscopy FTIRM: Fourier Transform Infrared Microspectroscopy GA: Gallic acid MDA: Malondialdehyde NIH: National Institutes of Health PC: Principal Component PCA: Principal Component Analysis Qc: Quercetin TBA: Thiobarbituric acid TBARS: Thiobarbituric Acid Reactive Substances.
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