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To investigate the influence of FWGE combined with cisplatin and 5-fluorouracil (5-Fu, both agents were purchased from Sigma-Aldrich, St Louis, MO, USA), HepG2, Hep3B and HepJ5 cells were treated with various concentrations of cisplatin and 5-Fu with 0.5, 0.25 and 1 mg/mL FWGE.
After incubation with various concentrations of FWGE (as indicated), cells were harvested by RIPA buffer (150 mM NaCl, 50 mM pH 7.5 Tris-HCL, 1% NP-40, 0.5% deoxycholate, 0.1% SDS, 1mM PMSF, 10 [micro]g/mL leupeptin, and 100 [micro]g/mL aprotinin).
CalcuSyn software (Biosoft, Cambridge, UK) was used for the statistical analysis of the half maximal inhibitory concentration (I[C.sub.50)] determined by MTT assay and for the combined effects of FWGE with chemotherapeutic drugs.
FWGE Treatment Induced Cell Death in Human Hepatocellular Carcinoma Cells.
For example, the viability of HepG2 cells following 0.5 mg/mL FWGE treatment was 85% as determined by MTT assay, whereas the result counted by a Scepter cell counter was 50%.
FWGE Enhanced Cytotoxicity of Chemotherapeutic Drugs on Human HCC Cells.
FWGE also effects cancer cell proliferation by inhibiting glycolysis.
FWGE affects a strong inhibition of transketolase (TK), slowing glucose uptake into pancreatic cancer cells and inhibiting the cells' synthesis of RNA.
FWGE also inhibits the enzyme glucose-6-phosphate dehydrogenase (G6PDFI), a metabolic enzyme essential for using glucose carbons to make ribose through the pentose phosphate pathway mentioned above.
As an aside, there is no evidence that FWGE is contraindicated with high-dose vitamin C (HDVC) therapy, which depends upon adequate levels of G6PD prior to commencement.
Both apoptosis and the inhibitory effects on glycolysis from FWGE can probably be explained by looking at the effects that FWGE has on mitochondrial function.
Hidvegi to provide the most effective FWGE formulation that will help people in need worldwide.
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