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FXL = [[alpha].sub.i] + [[beta].sub.1]FXD + [[beta].sub.2]FXLFXDR + [[beta].sub.3]DCLDCDR + [[beta].sub.4]ASFB + [[beta].sub.5]AS5LB + [[beta].sub.6][TRADE.EUR] + [delta][X.sub.it] + [[epsilon].sub.it] (1)
With the introduction of the Niton FXL field X-ray lab, we offer a range of products, both field-mobile and handheld, geared toward helping importers, brand owners and retailers implement a standardized inspection protocol for incoming shipments to verify compliance at all stages of the supply chain," says Marc Tremblay, vice president/general manager for Thermo Scientific Niton Analyzers.
The cells then were incubated for 15 min at room temperature, washed 3 times with cold 1 x PBS, and incubated with the trifunctional avidin-coupled nanospheres (20.0 g/L in 200 FXL binding buffer) for 15 min at room temperature.
Generally, we coated white polystyrene microtiter plates with 500-ng/well monoclonal antibody in 100 FxL coating buffer (50 mmol/L Tris-HCl, pH 7.8).
Aliquots of urine (25 FxL) were injected onto a Zorbax Bio series preparative GF 250 column (9.4 mm i.d.
We detected proteins in eluted fractions by profiling 1 FxL of each fraction on an NP20 array using Protein Biology System IIc MS.
After 2 washing rounds, we added 25 ng Eulabeled GAD65 in 75 FxL Delfia Assay buffer and continued incubation for 30 min on a Plate Shake.
The mixture was neutralized with 50[micro]L 0.2 mol/L Tricine and precipitated by adding 1 FxL (20 Fxg) glycogen (Invitrogen),15 [micro]L 3 mol/L sodium acetate and 375 [micro]L of absolute ethanol, followed by a 20 min incubation at 4[degrees]C.
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