Then, the membranes were incubated with various antibodies against specific proteins: HO-1, HO-2, and ferritin heavy chain (Cell Signaling Technology, Boston, MA, USA), ferroportin-1 (Santa Cruz Biotechnology, CA, USNFR-2 (Abcam), gp91 phox, UCP1, and UCP2 (Abcam), insulin receptor beta Cell Signaling Technology), insulin receptor phosphorylated at Tyr 1146 (Cell Signaling Technology) and at Tyr 1322 (Assay Design, Ann Arbor, MI, USA), and AKT, pAKT, AMPK, pAMPK, Mfn1, and Mfn 2 (Cell Signaling Technology) at 4[degrees]C overnight with constant shaking.
Specific primers for mouse ferritin heavy chain and hepcidin were used.
SOD-2, ferritin heavy chain, and GAPDH-HRP were purchased from Santa Cruz Biotechnology.
The membrane was incubated in cold conditions overnight with primary antibodies ([phospho-serine.sup.473]-AKT, AKT, [phospho-serine.sup.235/236]-S6, S6, p-ERTh/2, ERK1/2, p-P38, P38, phospho-tyrosin705-STAT3, STAT3, Bcl-2, Bax, SOD-2, and ferritin heavy chain 1 or GAPDH-HRP) in a 1: 1000 dilution with 5% BSA.
Postischemic treatment with Rapamycin also induced the expression of antioxidant enzymes (SOD-2, manganese-dependent superoxide dismutase, and ferritin heavy chain 1) in hearts as compared to Control and DMSO-treated I/R groups (Figures 8(b), 8(c), and 8(d)).
Analysis of mRNA transcripts of HO-1, transferrin, inducible NOS, ferritin heavy chain, and ferritin light chain was performed using qPCR.
The relative ferritin heavy chain, light chain, transferrin, and inducible nitric oxide synthase mRNAs levels in 15 copy number transgenic cloned pigs are significantly increased compared that of lower copy number of transgenic pig (Figures 3(c)-3(f); P < 0.05).
(c) Ferritin heavy chain, (d) ferritin light chain, (e) transferrin, and (f) inducible NOS.
Isolation and characterization of a cDNA clone for human ferritin heavy
The ferritin heavy
chain converts the more unstable form of iron (Fe2+ or the ferrous) to a more stable (Fe3+ or the ferric) form that can be the safely sequestered and deposited within the ferritin shell.
(2004) Ferritin heavy
chain upregulation by NF-kappaB inhibits TNFalpha-induced apoptosis by suppressing reactive oxygen species.
Their topics include therapeutic perspectives involving ferritin heavy
chain FtH, race and ethnicity, new findings about cardiovascular dysfunction, and Parkinson's Disease as a perfect target for gene therapy.