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Regulation of embryonic lung vascular development by vascular endothelial growth factor receptors, Flk-1 and Flt-1.
Paraffin coronal sections of the eyeball derived from different time points were incubated in primary antibodies including rat-specific vascular endothelial growth factor (VEGF, 1 : 150, Santa Cruz Biotechnology, Dallas, TX), rat-specific Flk-1 (1 : 150, Santa Cruz Biotechnology, Dallas, TX), rabbit-specific cAMP-response element binding protein (CREB, 1 : 150, Millipore, Darmstadt, Germany), and rat-specific PKC (1 : 150, Abcam, Cambridge, UK).
The monoclonal antibodies FlK-1 and VEGF (C-17) (1:100), secondary antibody Goat Anti-Rabbit IgG (H+L), Diaminobenzidine chromogen solution, streptavidin and peroxidase (rabbit ImmunoCrnz Staining System: sc-2051.) were obtained from Santa Cruz Biotechnology, California, USA.
reported that VEGF, Flk-1 and Flt-1 were differentially expressed in the endometrial epithelium and stroma during the proliferative and secretory phases (3).
Enuklee gozler histolojik analiz, kaspaz-3, kaspaz-8, kaspaz-9, Fas/Fas L, VEGF ve VEGF reseptorleri (Flt-1, Flk-1) ve TUNEL boyama icin hazirlandi.
Bautch, "The vascular endothelial growth factor (VEGF) receptor flt-1 (VEGFR-1) modulates flk-1 (VEGFR2) signaling during blood vessel formation," American Journal of Pathology, vol.
Circulating mature endothelial cells (CECs) may appear in the circulation by detaching from activated or damaged vessels and express CD34 and Flk-1, but, unlike the EPCs, they are negative for the haematopoietic marker CD45.9 CECs also express endothelial markers such as CD146 and CD31.
The number of cells positive for both Flk-1 and Sca-1 (Flk-1+/Sca-1+) and those positive for Sca-1 alone (Sca-1+) were measured by flow cytometry using an LSRII flow cytometer (BD BioSciences) and analyzed with FlowJo v8 software (Treestar Inc., Ashland, OR).
2 (murine flk-1); the receptor protein tyrosine kinases having the amino acid sequences shown in FIG.
A new communication system between hepatocytes and sinusoidal endothelial cells in liver through vascular endothelial growth factor and Flt tyrosine kinase receptor family (Flt-1 and KDR/ Flk-1).
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