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Dissociated cells were reacted with Allophycocyanin conjugated anti mouse Flk1 antibody (eBioscience, San Diego, CA), followed by sorting with a magnetic cell separation system (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany).
VEGF Receptor 1 (VEGFR1, Flt1) and VEGFR2 (KDR/ Flk1) are expressed on the cell surface of the human GBSCs.
Vascular endothelial growth factor D (VEGFD) is a ligand for the tyrosine kinases VEGF receptor 2 (Flk1) and VEGF receptor 3 (Flt4).
For cell sorting, live cells were harvested and double-stained with APC-conjugated FLK1 (1:100, BD Biosciences, USA) and PE-conjugated CXCR4 (1: 50, BD Biosciences, USA).
Cardiac precursor cells (CPCs) marked by type III receptor tyrosine kinase c-kit ([c-kit.sup.+]) with silenced Nox2 and Nox4 genes showed increased expression of the CPC stemness markers c-kit and Flk1 (receptor for vascular endothelial growth factor), while cells with overexpression of NOX2 and NOX4 presented decreased c-kit level .
Se ha descrito que la senalizacion sinergica entre Ihh y FGF2 provenientes del endodermo, promueve la expresion del receptor FLK1 (VEGFR-2) en el mesodermo adyacente.
Stanford et al., "A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis," Cell, vol.
However, at day 4, 30% of all differentiating NTC cells became FLK1-positive, while only 15% of the Fubp1 knockout cells had started to express FLK1. This difference became even more obvious at day 5 of differentiation (18% [FLK.sup.+] Fubp1 knockout cells versus 40% [FLK.sup.+] NTC cells), supporting the conclusion that FUBP deficiency in ESCs leads to delayed mesoderm differentiation.
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