The HTG EdgeSeq Lung Fusions RUO Assay is designed to measure and analyze mRNA ALK gene fusion events in formalin-fixed, paraffin-embedded
lung tumor specimens from patients previously diagnosed with non-small cell lung cancer.
According to the company, the companion diagnostic test approved by the US FDA for selecting TNBC patients for TECENTRIQ is the VENTANA PD-L1 (SP142) assay, which is an immunohistochemical assessment of the programmed death-ligand 1 (PD-L1) protein in tumor cells and tumor-infiltrating immune cells in formalin-fixed, paraffin-embedded
(FFPE) tumor tissue.
We constructed tissue microarrays from formalin-fixed, paraffin-embedded
tissue including 42 cases of cHL, 33 cases of NLPHL, 16 cases of anaplastic large cell lymphoma, and 7 cases of T-cell/ histiocyte-rich large B-cell lymphoma.
She and her colleagues at the Centers for Disease Control and Prevention in Atlanta developed the assay and evaluated it using RNA extracted from formalin-fixed, paraffin-embedded
placental/ fetal tissues from 53 women with varying pregnancy outcomes, and brain tissues from seven infants with microcephaly who died.
However, in tumor analysis, LCM is performed on formalin-fixed, paraffin-embedded
(FFPE) or cryo-fixed tissue to preserve its morphology.
FoundationOne CDx is a next-generation sequencing based in vitro diagnostic device for detection of substitutions, insertion and deletion alterations, and copy number alterations in 324 genes and select gene rearrangements, as well as genomic signatures including microsatellite instability (MSI) and tumor mutational burden (TMB) using DNA isolated from formalin-fixed, paraffin-embedded
(FFPE) tumor tissue specimens.
This study analysed protein expression and DNA alterations for the KRAS, CDKN2A, SMAD4, and TP53 genes by immunohistochemistry and next-generation sequencing in formalin-fixed, paraffin-embedded
tumors in 356 patients with resected pancreatic adenocarcinoma who were treated at the Dana-Farber/Brigham and Women's Cancer Centre in Boston, University of Rochester Medical Centre, and Stanford Cancer Institute.
Deamination effects in formalin-fixed, paraffin-embedded
tissue samples in the era of precision medicine.
Zhao et al., "RNA sequencing of formalin-fixed, paraffin-embedded
specimens for gene expression quantification and data mining," International Journal of Genomics, vol.
Since November 2015, CDC has been developing assays for Zika virus testing in formalin-fixed, paraffin-embedded
(FFPE) tissue samples.
Immunohistochemistry (IHC) was described in 1941, (1) but it was not until the early 1970s that anatomic pathology used labeled antibodies on formalin-fixed, paraffin-embedded
tissues for routine diagnostics.
DNA extraction from archival formalin-fixed, paraffin-embedded
tissues: Heat- induced retrieval in alkaline Solution.