Transcription patterns (mean [+ or -] SE; n = 5) shown by the number of mRNA copies of the eight studied genes (HR3, EcRB, Neverland, HR38, MET, SRC, Hb2, and RXR), relative to G3PDH
, across the adolescent instar in females exposed to TBT L (gray triangles), TBT H (black triangles), or the carrier solvent (open circles).
(1998) ADH-1 Monomorphic Monomorphic G3PDH
120 Monomorphic 100 [GPI-1,2.sup.*] Monomorphic Monomorphic [GPI-3.sup.*] Monomorphic Monomorphic [IDH-3,4.sup.*] 120 110 100 100 90 90 70 -- 40 -- [MDH-1,2.sup.*] Monomorphic Monomorphic [MDH-3,4.sup.*] 125 -- 100 100 -- 95 60 90 [ME-1.sup.*] Monomorphic 105 100 [ME-2.sup.*] Monomorphic 100 95 [SOD-1.sup.*] 140 115 100 100 40 Loci Gregg et Farrington et al.
Parsimony analyses recovered three minimum length trees from the cpDNA matrix, two from the ITS matrix, four from the PI matrix, and one from the g3pdh
Molecular markers: Allozymes.--The following 13 loci were interpreted: Acn, F16dp, G3pdh
, Idh, Mdh-1, Mdh-2, Me, Pgi-2, Pgm, Skdh, 6-pgd, Tpi-1 and Tpi-2.
The reaction conditions were optimized to favor amplification of SARS-CoV gRNA and sgRNA; thus, when they were present at high levels, amplification of G3PDH
The expression of glyceraldehyde-3phosphate dehydrogenase (G3PDH
) mRNA was used as a reference.
Once Ca + enters into the mitochondria, it is used by various enzyme systems, namely, Pyruvate DeHydrogenase (PDH), Glycerol-3-Phosphate DeHydrogenase (G3PDH
), isocitrate dehydrogenase, and oxoglutarate dehydrogenase, as a cofactor for Krebs cycle reactions, thus contributing to the maintenance of mitochondrial metabolism .
Expression stability of two housekeeping genes (18S rRNA and G3PDH
) during in vitro maturation of follicular oocytes in buffalo (Bubalus bubalis).
1997), using PCR primers for glyceraldehyde 3-phosphate dehydrogenase (G3PDH
) and interleukin 8 (IL-8) (Clontech Laboratories Inc., Palo Alto, CA).
Oligo(dT)-immobilized PP microplates (GenePlate-PP, AGCT), Yoyo-1 (Molecular Probes), reagents for PCR [Taq polymerase and EZ rTth RNA-PCR kit (Perkin-E1mer)], the K562 cell line (American Type Culture Collection), a 100-bp DNA ladder, phosphate-buffered saline, vanadyl ribonucleoside complex, rabbit globin mRNA, cell culture medium and appropriate antibiotics, buffer-saturated phenol, biotin-dATP (Life Technologies), fetal bovine serum (HyClone), biotin-dUTP, primers for glyceraldehyde-3-phosphate dehydrogenase (G3PDH
; Clontech), AttoPhos (JBL Scientific), Genius hybridization buffer, LumiPhos (Boehringer Mannheim), and supported nitrocellulose membranes (Optitran, Schleicher & Schuell) were purchased from the designated suppliers.
We used eight polymorphic enzyme systems: glyceraldehyde-3-phosphate dehydrogenase (G3PDH
1.9), Glycerol-3-phosphate dehydrogenase (G3PDH
, EC 184.108.40.206), Isocitrate dehydrogenase (IDH, EC 220.127.116.11), L-lactate dehydrogenase (LDH, EC 18.104.22.168), Malate dehydrogenase (MDH, EC 1.1.