However, it has been proposed that they just participate in transepithelial glucose movement but are not able to phosphorylate it into G6P
, because proximal tubules virtually lack hexokinase activity [20,21].
We determined MIC by using the Etest method with Muller-Hinton agar containing 25 mg/L G6P
During this time, glucose is phosphorylated to glucose-6-phosphate (G6P
), and the latter is then oxidized to 6-phosphate glucoronate in the presence of NAD.
The MIC of FOM was measured by the agar dilution method in the absence of G6P
, in the presence of 25 [micro]g/mL G6P
, and in the presence of 25 [micro]g/mL G6P
and 2 mM cAMP
For the determination of G6P
dehydrogenase (G6PDH) enzyme activity, we followed the protocol described in Bergmeyer and Grassl .
After 21 days, the expressions of albumin, AFP, CK18, CK19, G6P
, claudin, HNF4, and CYP2B6 mRNA were evaluated in the cells cultured on the 2D collagen films and in the 3D collagen scaffolds and compared to the control group.
The reaction was initiated by the addition of NADPH-generating system (containing 1.3 mM [NADP.sup.+], 3.3 mM G6P
, 3.3 mM Mg[Cl.sub.2], and 500 unit/ml G6PDH).
The incubation medium consisted of 0.1 M potassium phosphate buffer (pH 7.4) containing MA, cocktail probe substrates, and an NADPH-regenerating system (NGS, including 0.1 M G6P
, 1.0 U/ml G6PDH, and 10 mg/ml [beta]-NADPH) in a final incubation volume of 0.1 ml.
La G6PD humana (EC 18.104.22.168) cataliza el primer paso de la via de las pentosas, en el cual la glucosa 6 fosfato (G6P
) es convertida a 6-fosfogluconato (6FG) y el NADP reducido a NADPH7' 8; esta enzima tambien puede degradar glucosa o pentosas de los nucleotidos procedentes de la hidrolisis de los acidos nucleicos de la dieta, hasta CO2 y agua.
Patients were divided in three groups according to WHO which defines mild anemia as Hb 11.0-11.9 g/dl moderate s Hb 8.0-10.9 g/dl and Hb less than 8.0 g/ dl.16 Chemicals: 6PG G6P
GSSG NADPH EDTA and NADP+ were obtained from Sigma-Aldrich Co.
Glucose-6-phosphate dehydrogenase activity was assayed in the incubation medium containing 0.2 M Mg[Cl.sub.2], 0.025 M G6P
, 0.0075 M NDAP, and distilled water.
The ATP reacted with glucose in the presence of hexokinase to produce ADP and glucose-6-phosphate (G6P