Activity of G6PDH
was assessed by the method explained by (Tepperman and Tepperman, 1962)
What special care should people with G6PDH
The activities of MDH and G6PDH
were both increased (p<0.05) in the livers of the IUGR pigs, and choline supplementation decreased (p<0.05) MDH activity (Table 7).
Immunohistochemical markers for G6PDH
, 6PGL, 6PGDH, and NRF2 were assessed by a semiquantitative evaluation method as follows : 0: negative or weak immunostaining in <1% of the tumor; 1: focal expression in 1-10% of the tumor; 2: positive in 11%-50% of the tumor; and 3: positive in 51%-100% of the tumor.
We compared the activity of glucose-6-phosphate dehydrogenase (G6PDH
), which is present in the inner membrane of Pseudomonas and is related to glucose synthesis, to that of GMD .
Evaluations of activities of enzymes (ACP, ALP, ALT, AST, LDH, and G6PDH
) were carried out as described in the protocols of Randox kits used for the assay of each enzyme.
activity, TG, TC, VLDL, LDL, and HDL were measured spectrophotometrically by using commercially available diagnostic kits and reagents obtained from Randox Laboratories (UK).
For the determination of G6P dehydrogenase (G6PDH
) enzyme activity, we followed the protocol described in Bergmeyer and Grassl .
enzyme presented one polymorphic locus for H.
Considering that little is known about the enzymatic activity of native forest species' seeds during germination, and that this process is influenced by environmental factors, the objective of this study was to investigate the changes in [alpha]-amylase, P-amylase, and G6PdH
activities in M.
Hexokinase (HK; EC 184.108.40.206) and glucokinase (GK; EC 220.127.116.11) activities were measured as described by Enes using a reaction mixture containing 50 mM imidazole-HCl buffer ([p.sup.H] 7.4), 2.5 mM ATP, 5 mM Mg[Cl.sup.2], 0.4 mM NADP, 2 units mL1 G6PDH
and 1 mM (HK) or 100 mM (GK) glucose .
Phosphorylation in the 100,000 xg soluble cytosolic fractions was measured at 37[degrees]C, pH 7.4, by recording increasing absorbance (measured at 340 nm) in a well-established enzyme-coupled photometric assay containing glucose-6-phosphate dehydrogenase (G6PDH
), ATP, and NADP .