(65 000-Da molecular weight) and GAD67 (67 000-Da molecular weight).
5] per well were incubated in mAb-coated plates together with 1 unit/mL interleukin-2 (R&D Systems, Lille, France), and 10 mg/mL GAD65
and 10 mg/mL insulin B9-23 or C peptide, or with phytohemagglutinin as positive control and dimethyl sulfoxide (diluent) as negative control in immunoglobulin-free media for 40 h.
Recently, nonradioactive ELISA methods have become available commercially for GAD65
, IA-2, and ZnT8 (the last was approved in September 2014 by the US Food and Drug Administration for use in the US).
In the human brain, GAD65
performs an essential role: it makes 'neurotransmitters' - chemicals that pass messages between brain cells.
Stiff-person syndrome: Quantification, specificity, and intrathecal synthenthesis of GAD65
The Company's development project consists of the protein GAD65
for the treatment and prevention of autoimmune diabetes.
ZnT8 is more specifically expressed in insulin-containing secretory granules than GAD65
Nonradioactive assays, such as ELISA-base formats, are limited for most islet cell antigens (currently available only for GAD65
lalpha,25-dihydroxyvitamin D3 induces an autoantigen-specific 1-helper 1/T-helper 2 immune shift in NOD mice immunized with GAD65
The children participating in the trial have auto-antibodies against GAD65
and at least one other auto-antibody.
However, TrialNet concluded in its presentation that Diamyd and the active substance GAD65
may have an effect when given earlier in the disease process to prevent type 1 diabetes, in a different treatment regimen, or in combination with other drugs," Diamyd Medical's acting CEO, Peter Zerhouni, said.
GAD exists in two different isoforms, GAD65