Sandwich immunoassays were developed for VLP-1 (monoclonal antibody 3A8-1 as a capture antibody and rabbit antibody R3471 for detection), GAD67 (rabbit antibody Rb4043 as capture and Rb4610 for detection), and zygin (mouse monoclonal IG4.
Zygin, neuroserpin, and GAD67 were below the limit of detection of this method (<2.
r] 25 000 GAD67 3 Myelin- 4 associated oligodendrocyte basic protein Synaptotagmin I 3 5 2 3 3 2 Tubulin 2 5 2 2 1 2 [beta] 4 Zygin 3 Glycine 1 3 4 3 3 5 receptor [beta] Protein 1 5 1 kinase C and casein kinase substrate in neurons 1 Internexin 4 neuronal intermediate filament protein [alpha] Neuroserpin 3 1 Synaptobrevin 2 5 1 Neurogranin 1 Ovary Heart Pancreas Uterus Breast Cervix Protein VLP-1 2 4 Synaptosomal- 1 1 1 associated protein, [M.
pipiens glutamate decarboxylases GAD65 and GAD67, enzymes involved in the synthesis of the neurotransmitter [gamma]-aminobutyric acid (GABA).
In the tadpole diencephalon, the expressions of GAD65 and GAD67 were similar across all treatments (data not shown).
GAD67 mRNA levels were not affected by OP (1 nM; group O[P.
The inclusion of the well-characterized neurotransmitter enzymes GAD65 and GAD67 allowed us to address known hypothalamic pathways in addition to the relatively unknown mechanisms of the candidate molecules.
Both GAD65 and GAD67 mediate the synthesis of GABA, but their distribution in the brain suggests divergent functional roles (56).
Given that cotreatment with OP (1 nM) plus UV-B increases GAD67 mRNA levels and OP (1 [micro]M) plus UV-B advances metamorphosis, there may be a GABAergic component to the control of TSH and/or PRL release in the leopard frog.