In this paper, the potent antiproliferative effect of mitraphylline has been demonstrated in these two human brain tumor cell lines (glioma GAMG and neuroblastoma SKN-BE(2)).
In GAMG glioma line, the [IC.sub.50] value for mitraphylline is less than that obtained for cyclophosphamide and vincristine and very similar in SKN-BE(2) neuroblastoma, for the first doubling time (Table 1).
[IC.sub.50] value data of mitraphylline and patrons, cyclophosphamide and vincristine, for GAMG glioma and SKN-BE(2) neuroblatoma at the first doubling time (48 and 30 h, in that order) Mitraphylline Cyclophosphamide Vincristine ([micro]M) ([micro]M) ([micro]M) GAMG 20.05 50.85 30.43 SKN-BE(2) 12.28 8.05 10.05
Furthermore, its growth-inhibitory and cytotoxic effects on glioma GAMG and neuroblastoma SKN-BE(2) cell lines were studied using cyclophosphamide and vincristine as controls.
We have used two human brain cell lines, neuroblastoma SKN-BE(2) and malignant glioma GAMG.
Two cell lines have been used, SKN-BE(2) neuroblastoma (Interlab Cell Line Collection [ICLC] CBA, Genova, Italy) and GAMG glioma (Deutsche Sammlung von Mikroorganismen und Zellkulturen [DMSZ] Braunschweig, Germany).
In order to determine SKN-BE(2) and GAMG cell proliferation, different concentrations (5, 10, 20, 30 and 40 [micro]M) were evaluated.
(d) Protein expression of the candidate stem cell markers (ABCG2, CD44, CD95, CD133, ELF4, Nanog, and Nestin) and the differentiation marker GFAP as well as the microglia marker Sparc in primary GBM cells (pGC) and GBM cell lines (A172, GaMG
, and HF66) determined by Western blot.