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The incorporation of selenium into selenoproteins was analyzed by adding (75)Se to cells in the form of selenite (University of Missouri Research Reactor) as described previously (Ganyc et al.
Reaction conditions and analysis of gene expression levels were carried out as previously described (Ganyc et al.
A549 cells were treated with 0, 1, 2.5, 5, and 10 (iM AgNPs for 24 hr and harvested; then clarified cell extracts were prepared as described previously (Ganyc et al.