The Ct values were used to calculate the relative mRNA expression of PPARG compared to GAPD
(reference gene) using the [DELTA]Ct.
Meanwhile, the control effect of GA-PD is worse than the one of ZN-PD in jointly controlling three attitudes to the small values between GAPD
We obtained full-length Try-2 and GAPD
cDNA clones from American Type Culture Collection and MMP2, MMP7, MMP9, uPA, uPAR, and MSH2 clones from the Incyte Genomics IRAL cDNA clone library (Incyte Genomics).
In preliminary experiments, amplification factors were determined for specific target sequences Mus musculus glyceraldehyde-3-phosphate dehydrogenase gene 3' untranslated region (Gapd
, and Mus musculus porphobilinogen deaminase (Pbgd).
Two of the 33 loci scored (appendix), GAPD
and GOT1, were invariant across all populations and species.