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A putative hybrid individual would contain one copy of the GBSSI gene with the 15 base-pair deletion and one copy of the gene that does not contain the deletion at this locus.
For field surveys of individual plants, it would be too costly and laborious to sequence the GBSSI region from each specimen to determine presence or absence of the deletion.
Fragments of nuclear GBSSI were amplified using primers L1-f and L4-r.
DISCUSSION--The PCR-RFLP results for specimens Hammer 303 (collected in Marysee Prairie Preserve, Liberty County, Texas) and Hammer 328 (collected in Fort Boggy State Park, Leon County, Texas) show the predicted GBSSI restriction-fragment pattern for a diploid genotype with one copy of the locus contributed by a D.
demissum- [D.sup.d] AAAAAA Series according Ploidy to Hawkes (1990) Molecular markers DNA sequences Nuclear of GBSSI clades (a); COS (Spooner et markers, Cai et al., 2008), al.
Waxy protein or granule-bound starch synthase I (GBSSI), is a key enzyme for amylose synthesis in the wheat endosperm .
Phylogenetic utility and evidence for multiple copies of granule-bound starch synthase I (GBSSI) in the Araliaceae.
phylogenetic and fruit evolutionary analysis of the major groups of the paleotropical woody bamboos (Gramineae: Bambusoideae) based on nuclear ITS, GBSSI gene and plastid trnL-F DNA sequences.
A molecular phylogenetic and fruit evolutionary analysis of the major groups of the paleotropical woody bamboos (Gramineae: Bambusoideae) based on nuclear ITS, GBSSI gene and plastid trnL-F DNA sequences.
2007 A phylogenetic analysis of the grasses (Poaceae), with attention to subfamily Pooideae and structural features of the plastid and nuclear genomes, including an intron loss in GBSSI. Aliso 23:335-348.
The granule-bound starch synthase (GBSSI) gene in the Rosaceae: multiple loci and phylogenetic utility.
Rosaceae) is clarified by DNA sequence data from duplicated GBSSI genes.
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