GC-C-IRMSGas Chromatography-Combustion-Isotope Ratio Mass Spectrometry
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GC-C-IRMS DETERMINATION OF 5[alpha]A, 5[beta]A, AND 5[beta]P
The GC-C-IRMS analyses were conducted on a Finnigan Delta Plus Isotope Ratio Mass Spectrometer.
The linearity of the GC-C-IRMS response was determined by preparing seven solutions containing all three analyses at 2.5, 5, 10, 25, 50, 100, and 150 mg/L, respectively.
The linearity of the GC-C-IRMS response was assessed by least-squares linear regression.
GC-C-IRMS measurement of urinary steroids has become a potent analytical method in doping control, although scrupulous attention to detail is necessary.
Once the GC-C-IRMS method is validated to the point of full acceptance by the sport and legal community, we expect that athlete 3 could be classified as a user on one urine showing the pattern of a high T/E ratio, high testosterone concentration, and [[delta].sup.13]C values less than - 3 SD.
In conclusion, a fundamental issue in doping control is whether GC-C-IRMS techniques will resolve ambiguities in the interpretation of the T/E test.