Immunohistochemical staining showed the lesional cells were positive for CK 8/18, CK7, S100, GATA3 (diffusely weak), SOX10 (Fig 2c, 2d), CK 5/6, p40 (patchy) and were negative for GCDFP-15
, CD117, SMA, AR, ER, PR, Her2 and DOG1.
Immunohistochemistry of gross cystic disease fluid protein (GCDFP-15
) in 65 benign sweat gland tumors of the skin.
Positive for (a) GATA-3, (b) GCDFP-15
(patchy staining pattern), and (c) mammaglobin (scattered staining pattern).
, p63, and calponin also show positivity in some MASC tumors, and the latter are negative for ER, PR, and human epidermal growth factor receptor 2 (HER2).
In this patient, IHC of CK-7, GCDFP-15
, ER, PR, and CK 19 were positive and strongly suggestive of primary site in breast.
Diagnostic utility of mammaglobin and GCDFP-15
in the identification of metastatic breast carcinoma in fluid specimens.
Immunohistochemistry showed that the majority of tumor cells were positive for PAS diastase, toluidine blue, cytokeratin (CAM5.2, CK7), S-100 protein, and GCDFP-15
. From the above findings, a diagnosis of chondroid syringoma was made.
But immunohistochemical markers such as ER, PR, gross cystic disease fluid protein (GCDFP-15
), and differential expression of CK7 and CK20 can facilitate an accurate diagnosis as seen in the present report.
In immunohistochemical examination, more than 50% of epithelial cells showed a positive reaction with ER, PR, panCK and CK7 and a focal positive reaction with GCDFP-15
(Figure 4a, b).
Histology confirmed localization of adenocarcinoma with immunohistochemistry ER 90%, PgR 35%, CK7 3+, gross cystic disease fluid protein 15 (GCDFP-15
) 3+, and HER2 1+ (Figure 1).
A CT-guided biopsy of the pleural nodule revealed adenocarcinoma (Figure 9, A) positive for GATA-binding protein 3 (GATA3) (Figure 9, B), progesterone receptor (PR) (Figure 9, C), estrogen receptor (ER) (Figure 9, D), mammaglobin (Figure 9, E), and gross cystic disease fluid protein 15 (GCDFP-15
) (focal), but negative for TTF-1 (Figure 9, F) and p40.
Immunohistochemical studies were carried out on the cell block and core biopsy sections using antibodies TTF-1 (1:200; Leica), thyroglobulin (1:1000; Dako), ER (1:50; Dako), PR (premade; Leica), GATA3 (1:200; Cell Marque), GCDFP-15
(1:500; Leica), CD56 (Premade; Leica), synaptophysin (1:200; Dako), chromogranin (1:200; Cell Marque), pAx8 (1:100; Cell Marque), villin (1:100; Leica), CK 7 (1:400; Dako) and CK20 (1:200; Novocastra).