He also sought proposals of the business and industrial community in respect to composition and TORs of GCLC
Exercise induced upregulation of glutamate-cysteine ligase, catalytic subunit (GCLC
) and glutamate-cysteine ligase, modifier subunit (GCLM) gene expression in Thoroughbred horses.
has developed the technology and expertise for the mass production of human NK cells by successfully conducting a first-in-human clinical trial using healthy donor-derived allogeneic NK cells and by developing CAR-NKs to enhance anti-tumor specificity and efficacy.
Aldosterone significantly decreased the expression of GCLC
and Nrf-1 compared with the untreated control (Figures 7(a) and 7(b)), while it increased Nox4 expression (Figure 7(c)).
In BMM, IFN-[alpha] reprogramming was associated with increased expression of genes associated with nitric oxide (NO) production (ASS1, NOS1, and NAGS) and decreased antioxidants (SOD2, GCLC
, TXNRD1, and TXNRD3).
A) Nrf2 and its target gene glutamate cysteine ligase (Gclc
) expression in livers of W5 animals.
This finding is in agreement with the results obtained in solitary ascidians, which saw an increase in the transcription of genes for GCLC
, GCLM, GS, metallothioneins, phytochelatin synthase, and SODb in Ciona intestinalis after treatment with 10 [micro]mol [l.sub.-1] Cd[Cl.sub.2] (Franchi et al., 2011, 2012, 2014; Ferro et al., 2013).
For the amplification of regions capturing the GCLM (-588C/T) and GCLC
(-129T/C) polymorphic sites, we used primers as described by Nakamura et al.
The nuclear translocation of Nrf2 was associated with increased expression of Nrf2 target genes heme oxygenase 1 (HO-1, p < 0.001), glutamate-cysteine ligase catalytic subunit (GCLC
, p = 0.04) and glutamate-cysteine ligase regulatory subunit (GCLM, p <0.001) (Figure 4(c)).
Furthermore, accumulating data are showing that Nrf2 is the crucial regulator of apoptosis by activating antioxidant cascades, such as HO-1, GCLC
, and NQO1 [15, 16], which contribute to the suppression of apoptosis.
To further confirm whether Nrf2-downstream genes are involved, mRNA levels of HO-1, GCLC
, and MRP2 were analyzed (Figure 9).
Tan was able to activate Nrf2 and increase the expression levels of Nrf2 target genes, including glutamate-cysteine ligase catalytic subunit (GCLC
), NAD(P)H:quinine oxidoreductase 1 (NQO1) and hemeoxygenase-1 (HO-1), in a dose-dependent manner in HepG2 cells.