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Prenatal administration of ethanol shows that acute intoxication during GD8 induced long-term changes in the CNS of the offspring .
On the evening of GD8, dams were implanted subcutaneously with Alzet osmotic pumps (model 2004; Alza Corp., Palo Alto, CA, USA) following the manufacturer's recommendations.
On the evening of GD8, dams were implanted subcutaneously with Alzet osmotic pumps (model 1004; Alza Corp., Palo Alto, CA, USA) following the manufacturer recommendations.
On GD8, corresponding to the beginning of organogenesis in the fetus, rats were weighed and implanted subcutaneously with a miniature osmotic pump (model 1002; Alza Corp., Palo Alto, CA, USA), which delivered 25 BPA (Sigma- Aldrich de Argentina S.A., Buenos Aires, Argentina) or DMSO (99.9% molecular biology grade; Sigma-Aldrich de Argentina S.A.).
Age Control Dam body weight (g) GD8 203.7 [+ or -] 5.3 GD10 214.7 [+ or -] 5.1 GD20 279.8 [+ or -] 7.9 Implantations 12.2 [+ or -] 0.5 No.
Embryos are not large enough to leave a visible implantation-site stain until about GD8. All our mice were dosed from GD6 to GD15, with spring mice additionally dosed on GD0-GD5.
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- GD Rom
- Gdansk Institute for Market Economics
- Gdansk Shipyard