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GENBANKNational Genetic Sequence Data Base
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Because polymorphisms seen in a single isolate could potentially be a result of culture or sequencing artifacts, we selected the target sites based on polymorphisms that were shared by at least two SARS-CoV isolates published in GenBank at the time of this study.
However, after the initial isolation, GenBank played the crucial role of helping to determine whether this was a new disease or an old virus that had evolved a new pathogenicity.
Twenty-five that had matches in the GenBank non-redundant database were analyzed by Northern blot for enrichment of mRNA in myelin versus LSS.
List of soybean cDNA and genomic clones used as RFLP markers and their GenBank accession numbers.
''To insist on GenBank deposition would have consigned the Celera sequence data to trade secret status...We strongly feel that this would have meant a greater prohibition on scientific progress,'' Kennedy said.
Yet this data, like the data from GenBank and the newly sequenced human genome, is largely useless unless it can be properly organized, analyzed and integrated.
GenBank accession numbers of haplotypes sampled are provided.
The obtained sequences of PCR products (lengths of 232 bp, 286 bp and 1113 bp), after alignment with known sequences (GenBank: X59786.1, GenBank: X56120.1, GenBank: E04303.1, GenBank: E04303.1), allowed determination of the complete nucleotide sequence of the mink growth hormone gene - the total length of the established gDNA sequence is 1745 bp (submitted to the GenBank under accession number JX489617.2 and KR025486- KR025490).
The primer CYP749f (5'-ggtgctcctggtggctaaaag-3') is located at the 5' end of the CYP21P and CYP21 genes (nt 87412-87432; GenBank accession no.
It is easier to simultaneously send multiple requests to GenBank's database than to Celera's equivalent because Kim Worley, an enterprising researcher at Baylor University, recognized the problem and wrote code to fix it--something Worley says she wouldn't and couldn't have done for a private company.
Amplification and sequencing of cryptozoological DNA with "universal" PCR primers with broad specificity to vertebrate taxa and comparison with species in the GenBank taxonomic database is an effective means of discriminating otherwise unidentifiable large marine creatures.