The immunoblot analyses performed to investigate the effect of CA on SOX2 and GFAP
protein levels showed that also the amount of these two proteins was reduced in GBM treated cells compared to controls (P < 0.05 and P < 0.01, respectively) (Figs.
He also had heterozygous mutation in the 8th exon of the GFAP
gene M451I (c.1245G>A), leading to the diagnosis of AOAD
The slides were first blocked with Dako dual endogenous enzyme block and Dako serum-free protein block, incubated for 30 minutes with a rabbit polyclonal antibody to cow GFAP
(Dako) diluted 1:40, a rabbit polyclonal antibody to human CD105 (Thermo Scientific Lab Vision, Kalamazoo, Michigan) diluted 1:200, a rabbit polyclonal Sox2 antibody (Merck Millipore/Chemicon, Darmstadt, Germany) diluted 1:5000, or a rabbit polyclonal antibody to human vimentin (Abcam, Cambridge, Massachusetts) diluted 1:1500 in antibody diluent.
immunoreactivity in the hydrocephalic brain was stronger than in the control brain, both in number of cells and intensity (Fig 3).
We used two markers: IBA1 for microglia  and GFAP
for astrocytes .
Representative images of GFAP
immunoreactivity in the corpus callosum and hippocampus of air- and CAPs-exposed male (A) and female (B) mice at PND14, with (C) relative quantification of GFAP
in those regions and in the dentate gyrus, cortex, midbrain, striatum, and anterior commissure immediately adjacent.
: Glial fibrillary acidic protein MSC: Mesenchymal stem cell bFGF: Basic fibroblast growth factor BMDSC: Breast milk-derived stem cells EGF: Epidermal growth factor Masc: Mammary gland stem cell CNS: Central nervous system ESC: Embryonic stem cell CRT: Cell replacement therapy hBSCs: Human breast-milk stem cells iPSC: Induced pluripotent stem cell NSC: Neural stem cell FBS: Fetal bovine serum.
The limits of detection for human GFAP
and S100B were 0.045 ng/mL and 15pg/mL, respectively.
In group 2, there was a migration of GFAP
and OX-42 immunoreactivity from the cytoplasm to the nuclear compartment of the cells and a higher intensity of the marking.
The peripheral nerve repair process depends on Schwann cells, which synthesize and secrete important substances such as neuregulin-1 (Nrg1), GFAP
, and P0.
The increase of GFAP
marker count in the presence of retinoic acid and valproic acid is more than difference between control group and retinoic acid group.
Most cases are attributed to a mutation of the GFAP
(glial fibrillary acidic protein) gene, which causes abnormal function or gain of function in the GFAP