GFP-TTransparent Generic Framing Protocol
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The P- GFP-T fragment was digested with Xba I / Hind III enzymes, and then was cloned into pK-Ben plasmid cut with Xba I / Hind III, resulting in an inverted insertion of P-GFP-T fragment between the trpC promoter and Hind III site in the pK-Ben, yielding plasmid pK-Ben-GFP (Fig.