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GHBPGrowth Hormone-Binding Protein
GHBPGreen Heme-Binding Protein
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[35] both showed that GHBP was upregulated by GH and higher salinity in fish.
GHBP purified from natural sources (ProSpec) was spiked into the samples to produce final concentrations of 0, 10, 75, 250, and 500 [micro]g/L.
In plasma, 50% of hGH molecules are expected to be bound to GHBP (9).
Six- to eight-week-old BALB/c mice were immunized with 0.3 mg porcine GHBP emulsified in Freund's complete adjuvant.
First, the ELISA results indicated that AN98, but not the control antibody, specifically react with the porcine GHBP (Figure 1A).
The assay was designed to specifically measure 22-kD GH with no interference from Pegvisomant or GHBP. We achieved this goal by selecting high-affinity mAbs after careful epitope mapping.
Interference was tested for recombinant human GHBP (Protein Laboratories; Rehovot), biotin, triglycerides, and bilirubin (all from Sigma), hemoglobin (MP Biomed), red blood cells (Alba Bioscience), albumin solution from human serum (SCIPAC), human antimouse antibody (Hama 1, Hama 2; Roche), and rheumatoid factor (SCIPAC).
Each assay should specify its degree of interference by GHBP within its physiological interval (23).
[1] Nonstandard abbreviations: GH, growth hormone; WADA, World Anti-Doping Agency; GC, gas chromatography; rhGH, recombinant human growth hormone; IGF-I, insulin-like growth factor-I; IGFBP, insulin-like growth factor-binding protein; IFA, immunofunctional assay; GHBP, growth hormone-binding protein; P-III-P, procollagen III terminal peptide; PICP, C-terminal propeptide of collagen type I; and ICTP, C-terminal cross-linked telopeptide of type I collagen.
Plasma GHBP was assayed by size exclusion HPLC, using [sup.125]I-GH.
In women on oral estrogen therapy, the GH-binding protein (GHBP) concentrations may be higher (15, 16).
In addition, binding of GH to the GH-binding protein (GHBP) is a potential source of interference in GH assay systems, although Jan et al.