Abbreviations DM: Diabetes mellitus CVDs: Cardiovascular diseases T2DM: Type 2 diabetes mellitus GK: Goto-Kakizaki GLUT-2: Glucose transporter GPDH
: Glycerol-3-phosphate dehydrogenase NZO: New Zealand obese OLETF: Otsuka Long Evans Tokushima Fat SDT: Spontaneously diabetic Torri TGF-[beta]1: Transforming growth factor-[beta]1 ECM: Extracellular matrix PKB: Protein kinase B ECC: Excitation-contraction coupling SR: Sarcoplasmic reticulum RyR: Ryanodine receptor CICR: Calcium-induced calcium release SERCA2: SR [Ca.sup.2+]-ATPase2 NCX: [Na.sup.+]/[Ca.sup.2+] exchanger ENDO: Endocardial EPI: Epicardial.
In addition to upregulation of C/EBP[alpha], we found increases in several enzymes and lipid regulatory proteins (FABP4, PLIN4, PEPCK, GPDH
, and adiponectin) that are known PPAR[gamma] targets.
Glycerol 3-phosphate dehydrogenase (GPDH
) was assayed following a modified procedure .
R: 5'-GCCTGCCACTTTCCTTGTG-3'; Glycerol-3-phosphate dehydrogenase (GPDH
Compared to DMSO treatment (control), the treatment of 3T3-L1 cells with 100 [micro]g/mL HR or FR extract for 48 h resulted in 36% and 34% inhibition, respectively, of GPDH
Phosphoenolpyruvate is then converted to glyceraldehyde-3-phosphate, which is reduced to DHAP by glyceraldehyde-3-phosphate dehydrogenase and then to G3P by GPDH
Four out of 16 loci (Gpdh
, Got-1, G6pdh and 6Pgdh) were monomorphic in the three populations analyzed.
22.214.171.124), and glycerol-3-phosphate dehydrogenase (GPDH
Average numbers of GPDH
in age group over 50 is 23.3 [+ or -] 2.6 and in age group under 50 it is 23.6 [+ or -] 2.8 which indicates that there is no sign of statistical deference (P=0.76).
Glycerol-3-phosphate dehydrogenase activity (GPDH
) activity was measured using a GPDH
Activity Assay Kit (TaKaRa, Japan) following the manufacturer's instructions.
activity was below the detection limit of the assay at days 0 and 1.
Population genetics of the metabolically related Adh, Gpdh
and Tpi polymorphisms in Drosophila melanogaster.