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GRIP1Glutamate Receptor Interacting Protein 1
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Pools of genomic DNA samples from the parental lines (KNP [n = 5] and Landrace [n = 5]) of the F2 intercross were re-sequenced to detect SNP markers across the whole genome, including the GRIP1 gene (ENSSSCG00000000478), using the HiSeq2000 massively parallel sequencing technology (Illumina Inc.
For the pyrosequencing analysis of missense and silent mutations in the GRIP1 gene, we made a primer set and minisequencing primer to be used in polymerase chain reaction (PCR) (Supplementary Table S1).
GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.