All patients had GSUI as determined by a battery of standard tests, including comprehensive history and physical examination, multichannel urodynamics, urine culture and sensitivity, quality of life questionnaires, cotton swab tests, ultrasound to measure bladder neck mobility, and a standing cough stress test.
Recurrent incontinence occurred in three patients, who required further surgery At 5 years, two women had mixed incontinence and nine had GSUI. Eight of the 12 women who were not available for examination at 5 years returned questionnaires stating that they were dry and had received no further treatment.
After the PCR,15 [micro]L of PCR product was digested with GsuI (MBI Fermentas, Hamilton, ON) or MseI (New England Biolabs, Burlington, ON) as directed for 2 h at 37 [degrees]C.
The amplified PCR product obtained from nonmutant DNA with the A455E primers was 68 bp, and was cleaved to 61 and 7 by by GsuI. In the presence of an A455E mutation, an extra restriction site was created, yielding restriction fragments of 42, 19, and 7 by (Fig.