Primer sequences for amplifying the GYPC gene were: F: 5'-GGGGAATAAGGAAGCGAGTC-3', R: 5-CTGAAA CAACGGCAAACAAA-3'.
We applied the real-time quantitative PCR to identify the relative mRNA expression levels of porcine GYPC gene in eight different tissues.
Detection of polymorphisms and association analysis of the porcine GYPC gene
Sequence comparison of an identical PCR fragment from three pig breeds were detected one significant SNP (g.29625094 T>C) in exon 3 of the GYPC gene.
These results indicate that there is a likely association between the GYPC gene and the serum hemoglobin level, which should be confirmed in a larger population.
In our initial GWAS, the GYPC gene was identified .
In summary, the GYPC gene was expressed widely in eight tissues.