Identification and characterization of a Pantoea citrea gene encoding glucose dehydrogenase
that is essential for causing pink disease of pineapple.
* Glucose dehydrogenase
flavin adenine dinucleotide
Plasma from the whole-blood sample diffuses into and solvates the reagent layer, which contains glucose oxidase or glucose dehydrogenase
The new process first combines glucose with a bacterial enzyme called glucose dehydrogenase
. In the presence of a compound known as NADP, this enzyme transforms a glucose molecule into gluconic acid and attaches a freed hydrogen atom to NADP, forming NADPH.
The US Food and Drug Administration (FDA)1 has issued a second alert to warn of serious errors with certain blood glucose-monitoring strips that use glucose dehydrogenase
pyrroloquinoline quinine (GDH-PQQ) methods (1).
We had failed to appreciate the fact that the glucose dye oxidoreductase mediator reaction (glucose dehydrogenase
) as used in the Roche Accu-chek also measures galactose.
The various test methods employed by self-monitoring glucose devices used by diabetics at home, point-of-care analyzers, and laboratory glucose assays, she pointed out, all use one of the following test methods: glucose hexokinase, glucose dehydrogenase
pyrroloquinolinequinone (GDH-PQQ), glucose dehydrogenase
nicotinamide adenine dinucleotide (GDH-NAD), and glucose oxidase reaction.
A third enzyme (glucose dehydrogenase
), which frees up the cofactor for reuse, completes the mix.
Methods using glucose dehydrogenase
with NAD as co-factor (GDH-NAD), hexokinase or glucose oxidase are specific for glucose and do not exhibit interference as a result of interfering sugars.
Both techniques use the enzyme glucose dehydrogenase
. Because of the used flow rate of 1 [micro]L/min, the concentration in the dialysate reaches only partial equilibration and thus does not reflect the absolute concentration in the extracellular fluid.
The design consists of a dual-wavelength photometer and a glucose dehydrogenase