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The polyclonal antibodies used in the present study have been characterized as to their epitopic recognition of the H-K ring region of the brevetoxin type 2 molecule (20,21).
With synthetic brevetoxin analogues, the antibodies showed epitopic specificity for the H-K ring part of the molecule, the essential binding region for all type-2 brevetoxins (20,21).
The antibodies used in this study are specific for the last H-K rings of the PbTx type-2 backbone excluding the side chain (15), so these antibodies will also recognize PbTx metabolites.