HAS2Hyaluronic Acid Synthase 2
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The levels of cumulus cell expansion-related genes, including HAS2, TNFAIP6, PTX3, and PTGS2, were determined by quantitative real-time polymerase chain reaction (RT-PCR) in three replicates using a BIO-RAD CFX96 (BIO-RAD, Hercules, CA, USA) instrument.
Collectively, the gene expression data suggest that FTY720 treatment modulates the expression of transcription factors (JUN and ATF3) and proteins that may participate in the inflammatory response (HAS2 and LCN2) at the systemic rather than local level.
We explored the effects of MT3 on FDM in the guinea pig and the effects of MT3 treatment on mRNA and protein expressions for TGF-[beta]2 and HAS2 in the retina and choroid in the guinea pig.
In order to determine the mechanism of action of YE, the expression of mRNA coding for HAS2, the essential enzyme for the natural production of hyaluronic acid, (11) was assayed by quantitative PCR in these same normal human fibroblasts.
(69) has2 is expressed in cumulus cells, whereas has3 mRNA is expressed only in oocytes; has1 mRNA was not detected in COCs.
HA synthesis is catalyzed by a family of three HA synthases (HAS1, HAS2, and HAS3) that are multipass transmembrane enzymes.
Hyaluronan is synthesized in the plasma membrane by any one of three homologous enzymes (HAS1, HAS2, HAS3) known as hyaluronan synthases [31].
This high-molecular-mass HA accumulates abundantly in naked mole rat tissues, owing to a more robust synthesis by a protein called HAS2 and a decreased activity of HA-degrading enzymes.
The researchers were also able to identify the specific gene hyaluronan synthase 2, or HAS2, responsible for the development of HMW-HA.
The relative expressions of AGG, COL2A1, HAS-1, HAS2, and TIMP-1 were downregulated, while relative expressions of BCL-2, BAX, CAS-3, CAS-9, and MMP-3 were upregulated when the number of days of joint luxation was increased (Figures 1 and 2).
Putative role of hyaluronan and its related genes, HAS2 and RHAMM, in human early preimplantation embryogenesis and embryonic stem cell characterization.
The existing HAS2 CIS offered by ON Semiconductor is designed for Attitude and Orbit Control (AOCS) space applications.