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(28) After hASCs transplantation, AD mice models showed increased VEGF, GDNF, NT-3, and anti-inflammatory cytokine IL-10, while IL-1[beta] remained unchanged.
In this study, we hypothesized that the role of hASCs in inflammatory diseases may be influenced by miRNA expression patterns.
GO used to adsorb fibronectin and transforming growth factor-beta 3 was incorporated in pellets of hASCs to obtain a hybrid pellets of hASC-GO sheets (size = 0.5-1 mum) which promoted the hASCs differentiation to cartilage through increasing the interaction of cell with fibronectin and providing transforming growth factor-beta 3 efficiently.
2014), and 100 nM TBTC did not change the [H.sub.2][O.sub.2] production in hASCs (Llobet et al.
After differentiation and 8 days of maturation, 2-D hASCs qualitatively assessed with Oil Red-O staining (Figure 2, left) showed small amounts of lipid accumulation in most (multilocular) cells.
However, experiments over the last several years have tended to show that even hASCs may have pluripotent abilities.
The current study carefully analyzed the significance of FAK, ROCK, and ERK1/2 proteins in the adipogenic and osteogenic differentiation of hASCs. The key results demonstrated the reciprocal regulation of FAK and ROCK signaling in the interface of hASC osteogenesis and adipogenesis.
Duroux et al., "Transcriptional signature of human adipose tissue-derived stem cells (hASCs) preconditioned for chondrogenesis in hypoxic conditions," Experimental Cell Research, vol.
Recently, a study has revealed that USP7 is related to osteogenic differentiation of human adipose-derived stem cells (hASCs) .
Previous studies demonstrated that mesenchymal stem cells (MSCs), such as human adipose tissue-derived stem cells (hASCs) and human bone marrow mesenchymal stem cells (hBMSCs), were potential stem cell sources for the applications of cartilage tissue engineering approaches [6, 7].
In this study, human adipose stem cells (hASCs), multipotent hMSCs easily isolated from adipose tissue, were cultured in knitted and rolled 3D scaffolds prepared from PBS and PLA-PBS blends of 5 and 25 wt% PBS.
The authors thank Associate Professor Anders Elm Pedersen from the Department of Immunology and Microbiology, University of Copenhagen, Denmark, and Liselotte Rothmann Norup from the Flow Cytometry Core Facility at the University of Copenhagen, Denmark, for their help with the cell sorting of the L2T-positive hASCs. The authors thank Kasper With Nielsen for his help with the animals and Michelle Westergaard Kaijer and Lotte Kellemann Kristensen for their excellent assistance with histology, all from the Cluster for Molecular Imaging, University of Copenhagen, Denmark.
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