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EDTA whole blood samples from individuals without hemoglobin variants (HbAA, n = 50) and with HbAE (n=20) or HbAD (n=15) representing a range of Hb A1c values from 4%-12% were analyzed on the [ultra.sup.2] and IFCC RM at the University of Missouri.
An overall test of coincidence of least-squared linear regression lines was used to determine if the relationships between [ultra.sup.2] and IFCC RM Hb [A.sub.1c] results for HbAE or HbAD samples were statistically significantly different (P < 0.05) from the relationship for HbAA samples.
 Nonstandard abbreviations: Hb A1c, hemoglobin [A.sub.1c]; HbAA, hemoglobin AA; HbAD, hemoglobin AD Punjab; HbAE, hemoglobin AE; IFCC RM, the IFCC HPLC-capillary electrophoresis (CE) reference method for Hb [A.sub.1c].
The relative utility of the two protocols in demonstrating the presence of hemoglobin variants was investigated using samples containing HbAC, HbAE, HbAD, HbF, HbAS, and HbTyne (7) and showed superior identification for the 3.0-min version (Fig.
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