EDTA whole blood samples from individuals without hemoglobin variants (HbAA, n = 50) and with HbAE (n=20) or HbAD (n=15) representing a range of Hb A1c values from 4%-12% were analyzed on the [ultra.sup.2] and IFCC RM at the University of Missouri.
An overall test of coincidence of least-squared linear regression lines was used to determine if the relationships between [ultra.sup.2] and IFCC RM Hb [A.sub.1c] results for HbAE or HbAD samples were statistically significantly different (P < 0.05) from the relationship for HbAA samples.
 Nonstandard abbreviations: Hb A1c, hemoglobin [A.sub.1c]; HbAA, hemoglobin AA; HbAD, hemoglobin AD Punjab; HbAE, hemoglobin AE; IFCC RM, the IFCC HPLC-capillary electrophoresis (CE) reference method for Hb [A.sub.1c].
The relative utility of the two protocols in demonstrating the presence of hemoglobin variants was investigated using samples containing HbAC, HbAE, HbAD
, HbF, HbAS, and HbTyne (7) and showed superior identification for the 3.0-min version (Fig.