The methylation status of LC3A in the promoter region and exon 4 including 6 and 8 CpG dinucleotides, respectively, was analyzed in cancer cell lines H226, COLO677, H322, and H1975 as well as in normal cells HBEC by BS (Figure 2).
In line with this, we found decreased expression of LC3A in 4 out of 6 lung cancer cell lines compared to normal human bronchial epithelial cells (HBEC), which correlated to DNA methylation of LC3A in promoter region and exon 4.
Moreover, it is demonstrated that garlic could significantly inhibit candidal adhesion to HBEC
(Ghannoum 1990) and reduce biofilm formation in C albicans (Shuford et al.
The expression of the IL-8 gene in HBEC is known to be regulated through both message stabilization and transcriptional activation that is modulated by signaling pathways that include growth factor receptors (Khabar 2010; Standiford et al.
In the present study, we investigated the effect of [O.sub.3] stimulation on EGFR phosphorylation and its relationship with IL-8 expression in HBEC. We report here that the cytosolic tyrosine kinase Src can regulate EGFR activity, further modulating [O.sub.3]-induced IL-8 expression.
The human bronchial epithelial cell lines HBEC
(ScienCell, Carlsbad, CA, USA) and BEAS-2B (ATCC CRL-9609, Manassas, VA, USA) were cultured in F12K medium (Invitrogen, NY, USA) or LHC-9 (Invitrogen) supplemented with 5% fetal bovine serum, 100 U/mL penicillin, 100 [micro]g/mL penicillin, and 100 pg/mL streptomycin (Invitrogen) in a humidified incubator with 5% C[O.sub.2] at 37[degrees]C.
CONCLUSIONS: Akt activation plays a critical role in enabling As-transformed HBEC
migration and invasion by promoting ZEBl and ZEB2 expression.
To our knowledge, no study has systematically examined the effects of PM2.5 on human bronchial epithelial cells (HBECs
) in vitro and the possible molecular mechanisms that regulate the expression of TGF-[beta]1 and PDGF.
We found that rs1800797 was significantly associated with increased IL6 mRNA expression in primary HBECs
, lymphoblastic cells, and fibroblasts, which may at least partially stem from the increased gene transcription assessed using the luciferase reporter assay.
Additionally, we used an established in vitro model of primary human bronchial epithelial cells (HBECs
) [7, 8] to determine the suppressive effects of paeonol on increases in intracellular ROS, activation of the ROS-sensitive inflammatory signaling pathways, and the induction of interleukin-8 (IL-8), all of which are mediated by CS extract (CSE).
The A459 and BEAS-2B epithelial cell lines and primary human bronchial epithelial cells (HBECs
) express PAR-1, -2, -3, and -4 as judged by RT-PCR and immunocytochemistry .
We hypothesized that gene expression profiling may discriminate vanadium from zinc in human bronchial epithelial cells (HBECs