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Levels of serum IgG against 7 antigens (ESAT-6, CFP-10, MDP1, Ag85A, Acr, HBHA, and HrpA) before treatment (labeled as "before") and after treatment (labeled as "after") were analyzed by ELISA.
Norris, "Mycobacterial protein HbhA binds human complement component C3," Infection and Immunity, vol.
Caption: Figure 3: (a) Levels of serum IgG against HBHA and HrpA before treatment in smear at entry subgrouped between 1+ and 2+ and 3+.
Although HBHA has been known to be associated with EPTB and it can stimulate IFN-[gamma] production in individuals with latent TB infection, little is known about the role of HBHA in the differential diagnosis of EPTB and pulmonary (PTB) by the combination of humoral and cellular immune response.
Antigens: By using the plasmid of pET32a (+) the HBHA gene was expressed in E.
Subjects: A total of 165 TB patients (outpatients) and 133 healthy volunteers (age ranging from 20 to 55 yr) were selected randomly, in this study to investigate the role of HBHA in TB diagnosis.
PBMCs were cultured with HBHA (2 [micro]g/ml), phytohaemaglutinin (PHA 4 [micro]g/ml, positive control) and PBS (negative control) for four days (8).
After the optimal concentration of HBHA protein was determined, the serum from each patient of PTB or EPTB and healthy P+B- and P-B- subjects was tested by enzyme-linked immunosorbent assay (ELISA) (8).
For other antigens, after 100 [micro]L of each of 4 [micro]g/mL HBHA, 14 [micro]g/mL Ag85A, 6 [micro]g/mL Ag85B, 1 [micro]g/mL ACR, 8 [micro]g/mL MDP-1, 3 [micro]g/mL TDM-M, 3 [micro]g/mL TDM-K, and 0.5 [micro]g/mL LAM were coated, similar methods were conducted to detect their specific corresponding IgG [25, 26].
IgG Antibodies against Ag85B, Ag85A, ACR, HBHA, TDM-M, TDM-K, MDP-1, and LAM.
Lee et al., "Mycobacterium tuberculosis HBHA protein reacts strongly with the serum immunoglobulin M of tuberculosis patients," Clinical and Vaccine Immunology, vol.
Caption: Figure 2: IgG antibody responses to Ag85B, Ag85A, ACR, HBHA, TDM-M, TDMK, MDP-1, and LAM.
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