Take the diluted the PBNs solution to 5 mL with HBSS
without Ca2+ and Mg2+ and then invert the tube several times to suspend the cells.
Ecamsule was dissolved in HBSS
; the pH had to be adjusted to 7.0 by adding 0.1 N sodium hydroxide (approximately 2 [micro]l/ml).
Based on Hb electrophoresis diagnosis of the 47 participants, 18 participants (38.3%) had HbSS
, 13 participants (27.7%) had HbAS, 3 (6.4%) had HbS/[beta]-thalassemia (mild), 2(4.2%) had HbS/[beta]-thalassemia (moderate), and 11 (23.4%) had normal HBAA.
After rinsing off the ethanol with Hanks Balanced Salt Solution (HBSS
), bones were transferred to RPMI-1640 in a sterile Petri dish.
Cells were washed with HBSS
supplemented with L-glutamine and HEPES and intravitaly stained with DNA Nuclear Green DCS1 dye (Abcam) for 40 min at room temperature in the darkness, and washed twice with HBSS
Similarly, the matrix interference was also investigated using (i) PBS medium collected from release study control (dual-ligand NPs without any loaded drug) and (ii) uptake/transport study buffer (HBSS-P) comprising HBSS
containing 10 mM HEPES and 20 mM glucose (collectively the "experimental media").
Sections were deparaffinized, washed with HBSS
, permeabilized with 0.1% Triton X-100 (Sigma) for 10 min, and blocked with 1% Power Block (BioGenex) for 30 min at room temperature.
We carried out a descriptive comparative study of pregnant women in the age group of 15-40 years suffering from Sickle cell anaemia (HbSS
) in Government Medical College, Kozhikode from November 2014 to October 2016.
The epithelium sample was washed three times in 50 ml HBSS
buffer, and then incubated in 2 mg/ml collagenase H (Roche, Mannheim, Germany) at 37[degrees]c for 60 minutes.
If they could choose a better storage medium, they would use Hanks' balanced salt solution, an option that is well documented in the literature.18 Comparing milk with Hank's Balanced Salt Solution (HBSS
), authors noted that HBSS
demonstrated better results, even when the teeth were maintained for twelve hours.19
After ultracentrifugation the supernatant was discarded and the pellet containing the microparticles with MPO or iMPO was resuspended in Hank's balanced salt solution (HBSS