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There were significant differences in HBV DNA load, HBsAg levels, HBeAg contents, and ALT levels among IT, CHB, and AHB groups.
That is why in addition to continued qHBsAg testing, it is important to determine whether or not other testing, such as HBeAg status, liver enzymes (AST, ALT), liver function (ALB, INR, Bili), elastography, and portal vein and spleen size analysis, among others, may be appropriate.
The presence or absence of the double mutant was correlated with viral load, aminotransferases levels, as well as with HBeAg and anti-HBe parameters.
These data indicate that the decreasing prevalence of HBeAg was not due to the increasing age of treatment-naive patients but due to an authentic phenotypic change of HBV over the years.
Key Words: Chronic hepatitis B, HBeAg, Seroprevalance
This was explained by associated factors such as positive HBeAg and high baseline HBV DNA.
15 cells of each group cultured for 3 days as above, the culture supernatants were collected to determine the HBsAg and HBeAg secretions using the enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturer's protocol (Shanghai Kehua Biotech Co.
The stock of Elisa kits for HBeAg test expired on November 12 of 2014 but was used till March 3 of 2015.
HbeAg positive individuals with high viraemia need to be treated with antiviral drugs during the last trimester of pregnancy in order to prevent vertical transmission.
Dichas muestras fueron remitidas a la Coordinacion Red Nacional Bancos de Sangre, (CRNBS-INS) en donde nuevamente se realizo la tamizacion por inmunoensayo con la tecnica de quimioluminiscencia amplificada (Vitros ECI de Ortho Clinical Diagnostic Johnson & Johnson) para los siguientes marcadores de HB: HBsAg, anti-HBc, HBcIgM (anticuerpo IgM contra el antigeno central), anti-HBs, HBeAg, anti-HBe.
46% women were positive for HBsAg showing exposure to HBV and about 10% women in this HBsAg positive group were also positive for HBeAg showing highly infective stage with active replicating virus where chances of virus transmission to the newborn are almost 90% in pregnant women.
The subjects who had negative HBeAg, a HBVDNA value of <2000 IU/mL and normal transaminases were classified as inactive carriers, the subjects who had positive HBeAg, increased HBVDNA levels and normal or near-normal transaminases were classified as immunotolerant patients and the subjects who had positive HBeAg, increased HBVDNA levels, increased transaminase levels and inflammation and fibrosis on biopsy were classified as immunoreactive patients (8).
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- HBEAG-binding protein 2-binding protein C
- HBEBP2-binding protein C