After labeling, cultured HCAECs were fixed, and the incorporation of BrdU was quantified.
1A ) not only significantly enhanced the proliferation of cultured C2C12 cells and HCAECs up to ~ 2-fold, compared with DMSO control cells (Fig.
The subconfluent monolayers of HCAEC were exposed to interleukin-1[alpha] (IL-1[alpha], 10 ng/ml medium, Clonetics Cell Systems) for 1 day.
Flow cytometry was used to measure the expression of CAMs on the surface of the HCAEC before and after treatment with IL-1[alpha].
The effects of IL-1[alpha] and garlic extract plus IL-1[alpha] on the expression of ICAM-1 and VCAM-1 by HCAEC are shown in Fig.
The effect of IL-1[alpha] in the absence and presence of 4 mg garlic extract on the expression of ICAM-1 and VCAM-1 by HCAEC is shown in Fig.
Because it is unclear how NSAIDs affect the coronary artery endothelium, we set out to compare three different NSAIDs, specifically potent diclofenac, highly selective COX-2 inhibitor etoricoxib, and enolic acid-derived meloxicam on stimulated HCAEC.
Thus, the main aim of our study was to determine the impact of the aforementioned drugs used for therapy of systemic autoimmune diseases, such as RA, on inflammatory responses of SAA-activated HCAEC, suggesting their effects on the coronary artery endothelium.
Human coronary artery endothelial cells (HCAEC) were purchased from Cambrex BioScience (Walkersville, Maryland, USA).
Transcriptional Profiling of HCAEC Cells Exposed to Fasting or Postprandial Serum from Adult and Old Donors.
Repeated Exposure to Postprandial Sera Slightly Accelerates Cellular Senescence in HCAEC Compared to Fasting Sera.
This last result is consistent with our finding of an upregulation of cellular senescence and p53 pathway in the HCAEC model exposed to postprandial serum.