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Then, 0.3 mL of 0.5 M HClO4 was included in the mixture in order to precipitate the proteins.
Nitric acid is used commonly since it produces the fewest polyatomic interference in contrast to HClO4 and H2SO4.
Then shoots were rinsed with tap water and dipped into 70% alcohol for one minute and rinsed with HClO4 and distilled water.
Phosphate content in the plant was extracted with wet oxidation with HNO3dan HClO4  and the concentration of P was measured with spectrometer (Vitatron Scientific Instruments Dieren, the Netherlands).
Plant material (leaves) was wet-digested in nitric acid (HNO3) and perchloric acid (HClO4) which was in the ratio 0f 10:1 (V/V).
A known weight (0.5 g) of a dried powdered sample was digested using 3:1 mixture of concentrated HN[O.sub.3] (69-70.5%) and concentrated HClO4 (70%) under reflux at a temperature of 240[degrees]C for 2:30 hours.
Aliquots of sample were digested with a mixture of 4mL of HN[O.sub.3] (c) and 1mL HClO4 (Aldrich or Sigma Chem.
A uranium standard solution (100 ppm (w/v)) was prepared from analytical reagent grade uranyl nitrate hexahydrate in 3 M of HClO4. Arsenazo-III (0.07% w/v) solution was prepared in 3 M of HCl[O.sub.4].
Subsamples of the ground shoot (200mg) and root (100mg) were digested in a mixture of concentrated HNO3 and HclO4 (4:1 by volume) and the As, Cr and Cu in the digested solutions were determined by AAS.
On varying perchloric acid concentration from 0.1 to 0.5 mol[dm.sup.-3] the negative catalyzed kinetics was observed by the addition of HClO4 and first-order was evaluated (Table 3).
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