Specimens were tested for the presence of FLUAV and FLUBV, (16) HRSV (variant A and B), (17) HPIV species 1-4, (18) HRV species A and B (HRV-A/B), (19) HMPV, (20) HAdV, (21) HBoV species 1-4, (22,23) WUPyV and KIPyV, (24) and HCoV species OC43, 229E, NL63, and HKU125 by real time PCR assays.
Clinical samples of HRV-C, HCoV, KIPyV, WUPyV and HBoV, confirmed by PCR amplification and sequencing analysis, were obtained from patients with respiratory illness and used as positive controls.
1%) collected from 40 patients were positive for at least one of the viral pathogens screened for, namely 11 HAdV, 12 HBoV (four HBoV-1 and eight HBoV-2), seven HRV (4HRV-A/B and three HRV-C), two HMPV positive, two FLUVB positive, and one HRSV-A, HCoV (HKU1), and HPIV-3.
In the process, the investigators identified genomic sequences of a novel HCoV they called the New Haven coronavirus (HCoV-NH).
A comparison of the HCoV-NH with the HCoV identified in the studies from the Netherlands "revealed that these viruses are closely related and likely represent the same species," the investigators observed.
tested Dromedary contact 20/294 A, slaughterhouse workers A1, camel slaughterers Qatar 4/5 A2, sheep slaughterers Qatar 3/104 (contact with camels/ camel slaughterers) B, central animal market workers Qatar 1/8 C, barn workers at international Qatar 4/22 camel racing track D, camel farm workers Qatar 8/155 No dromedary contact 0/204 E, construction workers Qatar 0/56 F, sheep farmers Qatar 0/10 G, specificity controls G1, recent infection with a GER, NL 0/66 common hCoV
G2, suspected infection with NL 0/72 Bordetella pertussis Exposure type, cohort Serum samples tested by [PRNT.