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In Beijing city, RSV was reported to be the predominant virus in LRTIs in children, with a positive rate of 27%; PIV, MPV, and HCoV were not observed between 2004 and 2006 .
All samples were tested with Infiniti RVP plus assay on Infiniti Plus analyzer which identifies the following respiratory viruses: Influenza A virus (FluA), Influenza B virus (FluB), Influenza A-Swine H1N1 virus, hPIV (1,2,3, and 4), hRV (A and B), EV (A , B , C, and D), hCoV (HKU1 , OC43 , NL63, and 229E), hMPV (A and B), hRSV (A and B), and hAdv (A, B, C, and D) with 90% sensitivity and 100 specificity according to manufacturer (AutoGenomics, USA).
(14) Infections caused by other important viral pathogens such as HPMV, HRV, HBoV, and HCoV are not monitored.
In 2 out of the 7 HRV-C samples coinfections with hMPV or hCoV were identified and both were symptomatic.
"Population-based studies are required to define the burden of disease caused by this novel HCoV, and such studies could provide information on causality."
ADV, HBoV, HCoV, HMPV, IFV, RSV, PIV, EV, and HRV viruses were detected by conventional polymerase chain reaction (PCR) or reverse transcription- (RT-) PCR assay.
Seasonal influenza virus B (IBV), human rhinovirus (HRV), human metapneumovirus (hMPV), adenovirus (AdV), human respiratory syncytial virus (HRSV) and human coronavirus (HCoV) detections were performed as previously described.
tested Dromedary contact 20/294 A, slaughterhouse workers A1, camel slaughterers Qatar 4/5 A2, sheep slaughterers Qatar 3/104 (contact with camels/ camel slaughterers) B, central animal market workers Qatar 1/8 C, barn workers at international Qatar 4/22 camel racing track D, camel farm workers Qatar 8/155 No dromedary contact 0/204 E, construction workers Qatar 0/56 F, sheep farmers Qatar 0/10 G, specificity controls G1, recent infection with a GER, NL 0/66 common hCoV G2, suspected infection with NL 0/72 Bordetella pertussis Exposure type, cohort Serum samples tested by [PRNT.sub.90], no.
Human coronaviruses (HCoVs) OC43, 229E, NL63, and HKU1 are frequently associated with upper respiratory tract infection but can also cause lower respiratory tract infections (LRTIs), such as pneumonia or bronchitis.
In the past, human coronaviruses (HCoVs) found in both group 1 (HCoV-229E) and group 2 (HCoV-OC43) were associated only with mild upper respiratory tract diseases (5), whereas the novel SARS-CoV appears to be the first human coronavirus responsible for severe disease in humans.
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- hcp structure