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HEPESN-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic Acid
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Caption: FIGURE 2: Fluorescence response of B (1 [micro]M) in THF: HEPES (9.5: 0.5) (v/v) among various nitroaromatics and anions.
For the MB-AuNPs synthesis we used three concentrations of HEPES buffer [25, 50, 75 mM] as solution.
This clearly proves that, under HEPES, that is, nominally CO[sub]2 /HCO[sub]3 [sup]− -free, buffered solution, there is a Na [sup]+ -dependent, but CO[sub]2 /HCO[sub]3 [sup]− -independent, acid-extruding mechanism involved in the pH [sub]i recovery following induced intracellular acidosis in the HUASMCs.
Subsequently, the lungs were randomly divided into six groups; one control normoxic group (NOX, n=4) and five hypoxic groups of control (HOX, n=7), DIDS (400 [micro]M, n=3) treated, DIDS (200 [micro]M, n=5) treated, HEPES 2 (n=4), and HEPES 1 (n=4).
Estimated solubility of human osteoprotegerin in select solution formulations Solution Formulation Solubility (mg/mL) 50mM HEPES pH 7, 10mM NaCl 1.0 50mM HEPES pH 7, 0.2M NaCl, 0.1M L-Arg & L-Glu, 0.2M 4.5 trehalose 50mM HEPES, pH 7, 0.2M NaCl, 0.15M trehalose, 10% 11 glycerol Figure 5.
Every lot of materials/devices was tested with "clean" (free from albumin and [T.sub.4]) 0.01 mol/L HEPES buffer (pH 7.4).
C1.MC/C57.1 (C57) (Tsai 1996) mouse mast cells were cultured in DMEM supplemented with 10% heat-inactivated FCS, 4 mM L-glutamine, 25 mM HEPES, 50 [micro]M 2-mercaptoethanol, and 100 [micro]g/mL penicillin/streptomycin (complete DMEM).
The leftmost portion of the trace indicates the size of the differential signal recorded from one cell bathed in a skate-modified Ringer containing 2 mM of the pH buffer HEPES and adjusted to pH 7.60 with NaOH.
In separate experiments, cell samples were collected 72 hr after exposure either for GSH-R activity measurements or for culture in HEPES for 16 hr with or without cystine (24 [micro]g/mL) for CYSH and GSH assessment.
For measuring potassium, the Periplaneta Ringer of Smith was used (157 mM [Na.sup.+], 3 mM [K.sup.+] 2 mM [Ca.sup.++], 2 mM [Mg.sup.++] 165 mM Cl and 8.6 mM Hepes, pH 7.2).
Cells were collected by centrifuge at 600 x g for 5 min and homogenized in 20 mM Hepes, pH 7.8, containing 1.5 mM Mg[Cl.sub.2], 10 mM KCl, 0.5 mM dithiothreitol, and protease inhibitors [0.5 mM phenylmethylsulfonyl fluoride (Wako Co., Osaka, Japan), 5 [micro]g/ml pepstatin (Peptide Institute Inc., Osaka, Japan), and 5 [micro]g/ml leupeptin (Peptide Institute Inc.)].
The surface-to-volume ratio of the starvation medium--water or Hepes buffer--should be about 5 [cm.sup.-1] (or more) to ensure more than 95% oxygen saturation of the starvation medium.