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HEPESN-2-Hydroxyethylpiperazine-N'-2-Ethanesulfonic Acid
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sup-]free during the steady state period and at min 0 of the HEPES 1 and HEPES 2 groups.
5), Los testiculos que solo fueron inyectados con medio HTF con HEPES mas Azul de Trypan, no presentaron minitubulos (Fig.
The contents of all the tubes were made up to 1 mL with HEPES buffer I.
It was noted that positive results were not obtained at lower concentrations when using the HEPES buffer despite the three-fold higher estimate of PSA extraction efficiency over extractions using sterile water at the 4ng PSA/ml equivalence level.
The follicular contents were pooled into 50 ml tubes and allowed to sediment, the sediment was placed into HEPES buffered Tyrode-lactate medium (TL-HEPES-PVA; 114 mM NaCl, 3.
1) at 37[degrees]C, by addition of 40 [micro]L of 1200 mmol/L HEPES acid (Fisher Biotech) per milliliter of serum.
1 (C57) (Tsai 1996) mouse mast cells were cultured in DMEM supplemented with 10% heat-inactivated FCS, 4 mM L-glutamine, 25 mM HEPES, 50 [micro]M 2-mercaptoethanol, and 100 [micro]g/mL penicillin/streptomycin (complete DMEM).
For the validation of the performance characteristics of the ED-ID-LC/tandem MS procedure, we used the following materials: (a) 1 serum pool from blood donations by apparently euthyroid donors for imprecision, inclusive of ED; (b) HEPES buffer (52.
85 M (adult) or 1 M (fetal) sucrose with 10 mM HEPES, pH 7.
Detection of PCR products by SPR was conducted at 30 [degrees]C on a BIAcore X biosensor with a streptavidin-coated SA5 sensor chip using HEPES-buffered saline containing, per liter, 10 mmol of HEPES (pH 7.
The lysis buffer consisted of 50 mM HEPES, 150 mM NaCl, 1% Triton X-100, 1 mM PMSF, and 20 [micro] g/mL aprotinin, leupeptin, and pepstatin.
A small amount of buffer was added (1 [micro]1 per cm length of axon) which contained 10 mM Na acetate, 10 mM HEPES (pH 7.