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At 90% confluency, the hFOB 1.19 cells were stained using the Elastica van Gieson Kit (Merck Millipore, Product Code: 115974) to detect the presence of collagen and Alizarin Red staining (Sigma--Aldrich, Product Code: A5533) to detect the presence of calcium.
The PBMNCs were co-cultured with hFOB 1.19 in T175 cell culture flasks containing an OB basal culture medium DMEM/F12 at the ratios of 1 OC: 1 OB, 1 OC: 2 OBs, 1 OC: 4 OBs, and 2 OCs: 1 OB, respectively.
The PBMNCs were co-cultured with primary hFOB 1.19 at the ratios of 1 OC: 1 OB, 1 OC: 2 OBs, 1 OC: 4 OBs, and 2 OCs: 1 OB, respectively.
In the first step, we performed time-point optimization of OB differentiation and identified a suitable ratio for the co-culture of PBMNCs and differentiated hFOB 1.19.
In the current study, we obtained hFOB 1.19 (ATCC[R] CRL-11372[TM]) via biopsy from the limb tissue of a fetus from a spontaneous miscarriage.
Li et al., "Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line," International Journal of Molecular Medicine, vol.
PEMF exposure to T/C-28a2 cells, a line of human chondrocytes, and hFOB 1.19 cells, a line of human osteoblasts, mediated a statistically significant increase in [A.sub.2A] and [A.sub.3] ARs (Figure 4).
Corciulo et al., "Pulsed electromagnetic fields increased the anti-inflammatory effect of [A.sub.2A] and A3 adenosine receptors in human T/C-28a2 chondrocytes and hFOB 1.19 osteoblasts," PLoS ONE, vol.
Caption: FIGURE 3: [A.sub.2A] AR (a) and [A.sub.3] AR (b) density in bovine chondrocytes and synoviocytes, human synoviocytes, T/C-28a2 human chondrocytes, and hFOB 1.19 human osteoblasts in the absence and in the presence of PEMFs.
Caption: FIGURE 4: Effect of PEMFs on [A.sub.2A] and [A.sub.3] ARs expressed in T/C-28a2 human chondrocytes and hFOB 1.19 human osteoblasts.
In another study, hFOB osteoblasts responded to the AAO membrane by growing extensions into nanopores of 89 nm diameter, thus adhering tightly to the nanotopography while showing normal phenotype and morphology .
Unrestricted growth of human osteoblast hFOB 1.19 cells has been observed near CNT regions claiming assistance by CNTs surfaces to promote cell growth and proliferation.
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