HGAIHoward Goldstein Associates, Inc. (Superior, CO)
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PCR-RFLP: Equal quantity of PCR products were digested for 3h with BamHI, BstNI or HgaI (New England Biolabs, USA) at 37[degrees]C for BamHI and HgaI and 60[degrees]C for BstNI following manufactures instruction.
When the same samples were subjected to HgaI digestion, clear species discrimination was seen (Fig.
In conclusion, our data showed that HgaI based groES PCR-RFLP is a simple and rapid method for the identification and differentiation of M.