HGFS

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Healthy human gingival fibroblasts (hGFs) were isolated and cultured in Dulbecco's modified Eagle's medium (DMEM) (Life Technologies, Carlsbad, CA, USA) containing 100 U/mL penicillin and 100 [micro]g/mL streptomycin (Life Technologies), supplemented with 10% fetal bovine serum (Life Technologies) at 37[degrees]C in the presence of 5% C[O.sub.2], and passed every 3-4 days.
Next, hGFs were seeded (1.5 x [10.sup.4] cells/well) over the discs in DMEM + 10% FBS and incubated in a humidified incubator at 37[degrees]C and 5% C[O.sub.2] for 24 h to allow cell adhesion.
There are different sizes of the flakes with divacancy for the same defect structure: from Z1 to Z12 for zigzag HGFs and from A1 to A6 for armchair HGFs (the nomination was shown in Figure 1).
SG2 cells and SCDC2 cells or HGFs were cultured in a direct coculture system and indirect coculture system.
On the Primex membrane modified with the high concentration of GRGD, the number of cells attached for hGFs or hPDLs were significantly higher than that of unmodified or modified with low concentration GRGD [Figure 2]a, b, and [Figure 3]a.
Liverpool grew its number of HGFs by 56% from 2009-12 to 2012-15 (from 141 to 220) and now has the second-highest rate of such firms in England, behind only London.
HGFs were seeded in 96-well plates (10,000 cells/well) and incubated in serum-containing medium at 37[degrees]C overnight.
The extracts were prepared from the "3L" or "L" latex rubber bands in each well of standard 6-well plates (SPL, Pocheon, Korea) containing 3.5 mL of RPMI-1640 (Welgene, Daegu, Korea) for the L929 cells or DMEM (Welgene, Daegu, Korea) for the HGFs. The L929 cells (mouse fibroblast, NCTC clone 929, Korean Cell Line Bank, Korea) or HGFs [23] (HGF-1, CRL-2014, ATCC, USA) were plated at a concentration of 1 x [10.sup.4] cells per well in standard 96-well plates (SPL, Korea) in 100 [micro]L of culture medium and incubated at 37[degrees] C.
LPS-enhanced MMP activities of HGF and U937 macrophages, reducing in vivo gingival tissue degradation in periodontitic rats.
At an RA concentration of 100 [micro]g/mL, the repression level of CYP26B1 was approximately 15-fold in cultured primary HGFs obtained from a healthy volunteer in our previous study [72].
147), to avoid which we needed longitudinal evidence on firms that achieve high growth (HGFs) but also on those that do not (N-HGFs).