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El producto amplificado se sometio a digestion enzimatica con la enzima de restriccion Hinf I (7), separandose posteriormente por electroforesis horizontal en gel de agarosa al 2% en buffer Tris-EDTA-borato 1x.
Yang, "Relaxed stability condition and state feedback Hinf controller design for T-S Fuzzy System", Int.
Restriction enzymes and expected fragment sizes Restriction Expected DNA SNP Enzyme fragments M680I Hinf I Wild; 126 bp+234 bp Mutant; 360 bp M694V Hph I Wild; 195 bp+23 bp Mutant; 218 bp M694I Pag I Wild; 195 bp Mutant; 182 bp+13 bp V726A Alu I Wild; 360 bp Mutant; 320 bp+40 bp SNP: single-nucleotide polymorphism; bp: base pair Table 2.
Then, 10 [micro]L target insert fragments (about 1,500 bp) were digested by 3U restriction endonuclease Hinf I (TaKaRa) [13, 14] and Pst I (TaKaRa) [15] at 37[degrees]C for 2h.
TABLE 1: Primer sequences and genotype differences after Hinf I enzymatic digestion of MTHFR C677T polymorphism.
Balas, "Application of Hinf fault detection and isolation to a Boeing 747-100/200 aircraft," in Proceedings of the AIAA Guidance, Navigation, and Control Conference, vol.
With help of three four-base-cutting endonucleases, Hinf I, MspI, and Rsal on COI gene, Hashimoto et al.
Section 3 introduces a Hinf parameter ID technique developed for the time-varying system.
Following digestion with the restriction endonuclease Hinf I, the genotypes included CC (117 bp), CG (117 bp, 94 bp, and 23 bp), and GG (94 bp and 23 bp).
1 :Low 2:Med 3:High technology HINF: Lack of Information on 0: Not exp.